Description
This course is designed to give students an in-depth understanding of protein purification. Students will learn the physical and chemical properties of various protein purification media used in biomanufacturing and how those properties can be utilized for purifying proteins. Students will be engaged in real world applications relating to the chromatographic techniques involved in preparing media, packing columns, calibrating columns, and equilibrating columns. Students will learn how to design protein purification schemes based upon their knowledge of the intrinsic properties of proteins. All purified proteins will be validated using electrophoretic and spectrophotometric techniques.